Wednesday, September 12, 2012

The Jester's Blog: Are You Dependent?

The Jester's Blog: Are You Dependent?

Are You Dependent?


In my previous post from last year, ?Stay Sober, Close Women?, I covered most of the bases of why one is better off keeping substances OUT OF their body while attempting to meet and mingle with the opposite sex. It comes as no surprise how many human beings are sheep when it comes to addiction of some kind. And the interesting thing to observe for me personally is how one?s commitment to their addiction remains steadfast regardless of how much self-improvement jargon they swallow in this PUA subculture. Every day one shouts ?I?m gonna become a PUA!? with a sick desperation in their voice, a glass of liquor in one hand and a cigarette in the other. It?s not that different from the pride countless spectators give themselves when ?their? sports team wins. Did they help the team win? No, they weighed their couches down while filling themselves with crap. Dating Coach, David Wygant, who for those of you don?t know is the same fella Will Smith?s character was based off of in the film ?HITCH?, has a zero-tolerance policy when it comes to surrounding himself with drunken buffoons. In fact, I have even more respect for Dave after reading that because I am pretty firm about screening those I allow into my social circle, as well as my singles events in Los Angeles. Heavy users of (insert substance here) needn?t apply. But he goes even further and separates himself completely from the group the moment beer bottles start opening. Considering how many singles events I?ve hosted and attended that have had open bars, or at the very least some wine, that eliminates a huge percentage of what could be great opportunities. But again, you can only admire a grown adult who stays true to HIS preferences and doesn?t bend for anyone. And that?s especially difficult when you?re talking about friends with whom you share a history. So as I?ve stated many times before ? if you do in fact claim to be pursuing self-improvement, then challenge yourself to take that so-called ?liquid confidence? and reproduce it while completely sober. For until this is achieved the fact remains, you?re dependent.?

Source: http://thejestersblog.blogspot.com/2012/09/are-you-dependent.html

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Coalition Drops Opposition to Dow?s Genetically Engineered Crops

[unable to retrieve full-text content]The group, which included canners and other food industry members, said it was heartened by Dow?s response to its concerns.

Source: http://www.nytimes.com/2012/09/12/business/energy-environment/coalition-drops-opposition-to-dows-genetically-engineered-crops.html?partner=rss&emc=rss

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Molecular beacons light up stem cell transformation

ScienceDaily (Sep. 11, 2012) ? A novel set of custom-designed "molecular beacons" allows scientists to monitor gene expression in living populations of stem cells as they turn into a specific tissue in real-time. The technology, which Brown University researchers describe in a new study, provides tissue engineers with a potentially powerful tool to discover what it may take to make stem cells transform into desired tissue cells more often and more quickly. That's a key goal in improving regenerative medicine treatments.

"We're not the inventors of molecular beacons but we have used it in a way that hasn't been done before, which is to do this in long-term culture and watch the same population change in a reliable and harmless way," said graduate student Hetal Desai, lead author of the paper published online Sept. 5, 2012, in the journal Tissue Engineering Part A.

In their research, Desai and corresponding author Eric Darling, assistant professor of biology in the Department of Molecular Pharmacology, Physiology, and Biotechnology, designed their beacons to fluoresce when they bind to mRNA from three specific genes in fat-derived stem cells that are expressed only when the stem cells are transforming into bone cells.

Throughout 21 days of their development, the cells in the experiments remained alive and unfettered, Desai said, except that some populations received a chemical inducement toward becoming bone and others did not. Over those three weeks, the team watched the populations for the fluorescence of the beacons to see how many stem cells within each population were becoming bone and the timing of each gene expression milestone.

The beacons' fluorescence made it easy to see a distinct pattern in that timing. Expression of the gene ALPL peaked first in more than 90 percent of induced stem cells on day four, followed by about 85 percent expressing the gene COL1A1 on day 14. The last few days of the experiments saw an unmistakably sharp rise in expression of the gene BGLAP in more than 80 percent of the induced stem cells.

Each successive episode of gene expression ramped up from zero to the peak more quickly, the researchers noted, leading to a new hypothesis that the pace of the stem cell transformation, or "differentiation" in stem cell parlance, may become more synchronized in a population over time.

"If you could find a way to get them on this track earlier, you could get the differentiation faster," Darling said.

Meanwhile the stem cell populations that were not induced with bone-promoting chemicals, showed virtually no beacon fluorescence or expression of the genes, indicating that the beacons were truly indicators of steps along the transformation from stem cell to bone.

Beacons don't affect cells

Desai said the team took extra care to design beacons that would not alter the cells' development or functioning in any way. While the beacons do bind to messenger RNA produced in gene expression, for example, they do not require adding any genes to the stem cells' DNA, or expressing any special proteins, as many other fluorescence techniques do.

The team performed several experiments using the beacons in conventionally developing bone cells to make sure that they developed normally even as the beacons were in operation. While some scientists design RNA-based probes to purposely interfere with gene expression, this team had the opposite intent.

"You know that [.38 Special] song 'Hold on Loosely but Don't Let Go?'" Desai said. "That's sort of the theme song for this. There's a set of rules for interference RNA, and we essentially did the opposite of what those rules said you should do."

Toward quicker healing

Now that the beacons' performance in indicating milestones of stem cell differentiation has been demonstrated, Darling said, the technology can be applied to studying the process in a wide variety of cells and under a variety of other experimental conditions.

In the case of tissue engineering, he said, the beacons can aid experiments seeking to determine what conditions (inducing chemicals or otherwise) are most effective in converting the most stem cells to desired tissues most quickly. They could help tissue engineers learn the best timing for adding an inducing chemical. They might also provide a way for tissue engineers to identify and harvest only those cells that are converting to the desired tissue.

"They are becoming bone cells and if you enrich for them and you get rid of all the ones that aren't becoming bone cells, it stands to reason that you will have a better product at the end," Darling said.

More broadly, Darling added, molecular beacons are proving useful in a wide variety of gene expression studies.

"The reason we are looking at this technique is that we wanted something we could use on any cell, look at any gene and not affect that cell while we are looking at it," Darling said. "If this is acting as we believe it is, we can really look at any gene that we want. It seems like a very versatile tool."

In addition to Darling and Desai, the other authors are Indu Voruganti, Chathuraka Jayasuriya, and Qian Chen.

The National Institutes of Health provided funding for the research.

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Story Source:

The above story is reprinted from materials provided by Brown University.

Note: Materials may be edited for content and length. For further information, please contact the source cited above.


Journal Reference:

  1. Hetal V. Desai, Indu S. Voruganti, Chathuraka Jayasuriya, Qian Chen, Eric M. Darling. Live-Cell, Temporal Gene Expression Analysis of Osteogenic Differentiation in Adipose-Derived Stem Cells. Tissue Engineering Part A, 2012; 120905121132000 DOI: 10.1089/ten.tea.2012.0127

Note: If no author is given, the source is cited instead.

Disclaimer: This article is not intended to provide medical advice, diagnosis or treatment. Views expressed here do not necessarily reflect those of ScienceDaily or its staff.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/top_news/top_health/~3/AHm_lLt--js/120911125341.htm

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Lily Collins Talks 'Insane' Actions Shots In 'Mortal Instruments'

Star tells MTV News what it takes to bring the fantasy novel to life on the big screen.
By Kara Warner, with reporting by Josh Horowitz


Lily Collins
Photo: MTV News

Source: http://www.mtv.com/news/articles/1693557/lily-collins-mortal-instruments-action.jhtml

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Prior Knowledge: A Predictive Database For Developers

PKlogoPrior Knowledge debuted its predictive database for application developers today at Disrupt San Francisco 2012. Based upon work first done at MIT, the service is offered through the company's Veritable API, which the company says is designed to make applications smarter. The technology uses state of the art machine learning behind the scenes to handle the complexity for real world data.

Source: http://feedproxy.google.com/~r/Techcrunch/~3/VxHvSutOVF4/

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Saturday, July 28, 2012

Why Build New: Escape Competition - Idaho Real Estate

New Construction HomeAre you tired of fighting all the multi-offer battles to get into an existing home? ?In today?s market this is a new reality, but you don?t have to try to beat other full price offers--you can go in a different direction where you are in control. This is an especially good time for building Boise new homes rather than buying an existing home, here?s why:

Low Inventory

Not only can it be frustrating to search and search for the right existing home and not find one, it costs you time and money. ? There are usually about twice as many homes on the market, but with many homeowners under water less are able to put their home up for sale. ?This creates more competition, drives prices up, and allows for less variety and options for homebuyers in every price range. ?Say you want a house with a pool in a certain school district, likely, you could have found it a few years ago, but there either isn't one for sale or it will be very hard to get today. ?Why limit yourself, when you can get just what you are looking for in Boise new homes.

Building in Boise

When building Boise new homes was a bit more popular, price for construction and lots was more expensive. ?Today, builders are making it possible to buy a new home for close to the same price as an existing one and they take little time to construct. ?This assures that you will get the home with the price, location, features, and timing that you are looking for. ?You won?t have to be unsure when to put your home on the market or anything else about your housing situation. ?Boise new homes give you the chance to customize everything--carpet, door handles, cabinets, and more. ?Simply put, it?s impossible to find an already built home that has exactly what you want; though it?s completely believable, even expected, that you will do so in your new construction home. ?Additionally, builders specialize in constructing homes in a certain location and price range, so even lower-end buyers can find a great builder for them. ?

The median home value in Ada County began rising in 2012 and continues to climb nearly every month. ?So, this is an unprecedented time to build a home for thousands less than it could cost in a couple months or years. ?By buying Boise new homes while they still are very inexpensive, you will see your Real Estate purchase appreciate, making you money just from your good buying decision. ?Get pre-qualified for a home loan to see how much home you can buy. ?Talk to a Hughes Group Real Estate professional to learn all you need to know about new home building. ?They?re sold many Boise new homes, and know the ins and outs well enough to give you peace of mind and confidence, even if you?ve never built before. ?They will get you in touch with a suitable builder and, as your representative, they will walk you patiently and expertly through every step of the fun home building process.

As lots fill up and buying Boise new homes increases in popularity, this window of opportunity can diminish. ?Homes today are built better than ever--up to date with today?s energy and construction standards. ?2012, a year for small, but noticeable economic growth, is a wonderful time to begin building before it becomes too expensive.

Source: http://www.buyidahorealestate.com/blog/why-build-new-escape-competition.html

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Friday, July 27, 2012

Millionaire medalists: Does Olympic spirit live on?

John Makely / NBC News

As a member of the U.S. basketball team, Ray Lumpp won gold at the 1948 Olympic Games in London. Lumpp played in an era where amateurism was central to the Olympic ideal. "Even if I just wanted to play professional basketball ... just by saying I wanted to be a pro, I would have been removed," Lumpp said. "The Olympics was supposed to be for fun and games -- no compensation. I was a gold medal Olympic champion, but I owed money."

By Ian Johnston, NBC News

Updated at 10:05 a.m. ET: LONDON -- On August 14, 1948, Ray Lumpp stood in London's Wembley Stadium. As "The Star-Spangled Banner" played and "Old Glory" fluttered in the breeze, an Olympic gold medal was placed round his neck.

"To be in the Olympics was a dream come true," Lumpp, 89, told NBCNews.com from his Long Island home. "To receive a gold medal ? it still shines in my heart."?

In the aftermath of World War II, parts of London still lay in ruins, food was rationed and the ?strictly amateur? athletes were put up in basic accommodation. But ?The Austerity Games? remain a special event for people like Lumpp.

In sharp contrast to 1948, the London 2012 Olympics has a total budget in excess of $17 billion, a sum greater than the GDP of many of the 200-plus competing nations. About 9 million tickets have been sold and a global TV audience of billions is expected to watch more than 10,000 athletes compete.

Sixty-four years after competing in London, four gold-medal-winning athletes recall the excitement of the summer Olympics

Amid the glorification of multi-millionaires competing in sports including basketball, tennis and soccer, the sea of corporate sponsorship and fortress-style security -- has the Olympic spirit been forgotten? What would previous Olympians make of today?s event?

Would the Ancient Greeks --? who staged the first Olympic Games in 776 B.C. -- give their blessing or call down the wrath of Zeus? And what would the founder of the modern Games, French aristocrat Pierre de Coubertin, make of the demise of the amateur ethos?


Courtesy of Ray Lumpp

Ray Lumpp, seen here in 1948, is due to travel to London to attend this summer's Olympics.

Lumpp played in an era where, unlike today, amateurism was central to the Olympic ideal.

?Even if I just wanted to play professional basketball, I would have been removed from the American team -- just by saying I wanted to be a pro, I would have been removed,? Lumpp told NBCNews.com. "The Olympics was supposed to be for fun and games -- no compensation."

"I was married with one child and one on the way. I was a gold medal Olympic champion, but I owed money,? he said.?"You couldn't have sponsors, you couldn't do this, you couldn't do that ... you have to live and you have to eat."

More London 2012 coverage from NBCNews.com

After Lumpp returned to the U.S., he signed a professional contract with the New York Knicks and was soon ?out of hock.?

'For the love of it'
While he said that the acceptance of professionals into the games was a good idea -- meaning countries could send their best competitors and a level playing field for all ? he added that "sometimes money is too important, you lose the ideals of the Games."

The soccer superstar tells Meredith Vieira about his long-time friendships with Princes William and Harry, and explains his fond feelings for Queen Elizabeth II.

?The Games are about taking part, peace and understanding, and competing against one another, not fighting ?? playing against each other for the love of it,? he said.

"[In 1948] we had great admiration for the British people. Whatever they had, that was it ? but whatever they had, they shared it and put on a great Games under the conditions,? he said.

Fortress London: UK protects Games with biggest security operation since WWII

Lumpp said the success of the 1948 Games ? the first since Munich 1936 in Hitler?s Germany -- had been vital.

"After that 12-year period when there were no Olympics, it was important, very important, that the next Games be a success because it would affect the future of the Games ? because people might say 'it's not worth it,' and it could fade away,? he said.

As the U.S. women's soccer team kicked off a game against France, one athlete from Greece was removed after sending what officials are calling a racist tweet. A further warning to athletes: the World Anti-Doping Agency said more than 100 athletes caught doping were sanctioned in the months leading up to the Olympics. NBC's Kevin Tibbles reports.

The gap between Games was somewhat longer when Coubertin hit upon the idea of recreating the evemt.

The Christian Roman Emperor Theodosius had decreed in 393 A.D. that ?pagan cults? such as the Olympic Games would no longer be permitted. Some 1,503 years later, the first modern event was held in Athens.

Modern Games born from war
Despite the emphasis on promoting global harmony, Coubertin?s big idea was born out of a war.

"It may be a little bit disappointing. You may think it's a product of peace,? Dikaia Chatziefstathiou, an expert on the Olympics and an academic at Canterbury Christ Church University in England, told NBCNews.com.

France had not long been defeated in the 1870-1871 war against Prussia and there was concern that the country?s youth were ?not very active,? she said. ?The French government worried that the army wasn't strong enough.?

Coubertin, an expert on education, was brought in to shake things up and, on a fact-finding mission to England, he noted the emphasis on studying Ancient Greece and Rome at the country?s private schools, and was also impressed by the emphasis on sport and ?muscular Christianity.?

This, he thought, could be the answer to France?s diminished military might.

The U.S. Olympic committee has asked the Navy SEALs to train athletes with about a dozen teams, including the women's field hockey team and swimmer Michael Phelps. Working with the elite warfare unit pushes the athletes to go beyond what they think they're capable of doing. NBC's Chris Jansing reports.

But the late 19th century was also the so-called Age of Optimism when it was hoped that the world could put an end to war, disease and other great scourges. International movements such as the Scouts, the international Esperanto language, the YMCA and others sprang up "all about making society and the world a more peaceful place,? Chatziefstathiou said.

"He [Coubertin] came to the idea that actually sport can be used to have a peaceful celebration among the nations because he saw the power of sport,? Chatziefstathiou said. "He said 'Why not use sport and education to actually unite nations around the world?'"

London's Olympic lanes befuddle motorists

The idea caught the world?s imagination, but the first Olympiad in 1896 was a very different games to 2012 or even 1948.

There were no women. ?He [Coubertin] really didn't want women to sweat. He didn't want women to have any physical exertion,? Chatziefstathiou said, explaining this in terms of the social norms of the aristocracy of the time.

For the first time ever, all 205 countries competing in the Olympic games are sending female athletes. NBC's Meredith Vieira reports and speaks with sprinter Tahmina Kohistani, the sole woman on Afghanistan's Olympic team.

Also, most of the 1896 competitors were members of the upper classes and, if the right sort of person turned up, they just might find themselves allowed to take part.

George Stuart Robertson was one such athlete. He wrote an Ancient Greek ode that was recited at the end of the 1896 games and won a bronze medal in the doubles tennis. He also took part in the discus, which was perhaps a mistake, as he is still on record as achieving the worst-ever throw of about 27-and-a-half yards.

London Stereoscopic Company / Getty Images

Crowds walk around the Olympic Stadium in Athens during the 1896 Summer Games.

However -- in a sign of the Olympics? ability to break barriers -- one of the heroes of 1896 was a Greek peasant called Spyros Louis, winner of the marathon.

?He became a big symbol of the Games ? because without money, without preparation he came and ran in his traditional [Greek] clothing,? Chatziefstathiou said.

While Coubertin?subscribed to amateurism, she said she did not think he would not be appalled by the money in today?s Games. "If he saw that the movement wouldn't really survive without commercialism ? I don't think he would be against commercialism with controls,? she said.

Chatziefstathiou?s interest in the Games extends beyond the purely academic. She will be one of scores of dancers from all over the world in Friday's Opening Ceremony and was enthused by the "joy" among them at a practice held Monday.

Oscar-winning director Danny Boyle, who is directing this year's Olympic Opening Ceremony, pulls back the curtain on rehearsals to reveal to Meredith Vieira what viewers can expect, including whether Queen Elizabeth II will make a special appearance.

Even Twitter keeps Opening Ceremony (mostly) a secret

"If Coubertin came back [today], he would absolutely love the spirit of the people, and how many people of all ages, all nationalities are all there and enjoying it, and really actually believing it [the Olympic spirit],? she said.

Concerns over corruption, such as betting scandals, might be a worry, but Coubertin would be proud of how ?his baby? had grown, she said.

"He wouldn't say 'Oh my God, this is a monstrosity' because he was so keen to keep the movement going,? Chatziefstathiou said. ?I think he would be absolutely over the moon."

East London, which will host the Olympic Games, boasts a colorful history. NBC News' Jim Maceda reports.

The participation of women, however, might prove too big a step for a man of his background, she suggested. ?I don't think he would like this. He would be able to adapt to many things, but this is a spectacle I don't think he would be keen to see."

Ancient Games: Naked and men-only
Most Ancient Greeks were similarly against women at the Olympic Games, and to a much greater degree. With the exception of the priestess of Demeter, who oversaw events for religious reasons, any woman found watching the events faced being killed.

However, at least one female spectator is said to have survived the experience.

Kallipateira, the mother of a boxer, sneaked in dressed as a man to watch her son compete, Armand D?Angour, a fellow and tutor in classics at Jesus College, Oxford University, told NBCNews.com.

?Then when her son wins, she jumps up with delight and gives herself away as a woman,? he said.

Check out our?'TODAY in London' blog

Summoned by the judges, she?told them how sport was part of her and her family's life, saying ?this is who I am.? And the judges, D?Angour said, decided to let her off.

The idea of female athletes would have been shocking for most Greeks, ?apart from one city state, which was Sparta,? he said.

The Trustees of the British Museum

This marble statue of an athlete stooping to throw the discus is one of several Roman copies made of a lost bronze originally crafted in the 5th century BC by the sculptor Myron.

In Sparta, women had a degree of equality and were known to be ?very sporty.?

?Spartan women were considered to be women with six-packs ? ?strong, not necessarily beautiful, and quite scary,? D?Angour said.

However, all Ancient Greeks would have been more in tune with the today?s Olympics when it came to ideas about money.

D?Angour said athletes were sponsored by their cities and spent years in training.

Tony Duffy / ALLSPORT, Getty Images

How has life treated the many U.S. Olympians who have dazzled and inspired us over the years? Find out in this handy then-and-now roundup.

?And of course if they won, they were feted, celebrated and odes were written for them ? an expensive business. They would be fed at public expense for the remainder of their lives. There was a lot of money in it,? he added.

Flame 'nothing to do with Ancient Greece'
The amateur ideal or so-called ?Corinthian spirit? was ?a bit of an invention really,? D?Angour said.

Other modern inventions include the Olympic flame ? ?that?s nothing to do with Ancient Greece, it comes from the idea of the eternal flame in Rome? ? and the Olympic rings, he said.

D?Angour, author of Ancient Greek odes to the Athens and London Olympics, said Ancient Greeks would be shocked by ?the completely irreligious? nature of the modern games.

?Zeus, the head of their gods, was very much in the center of the games,? he said. A central message was ?as great as human beings strive to be, they can never be as great as the gods.?

The Trustees of the British Museum

This large mosaic of Hercules, the legendary founder of the Olympic Games and patron of athletes, dates from the Roman period.

And they might also be disappointed that the athletes were wearing any clothes.

?They competed naked ? you?d see a lot of dangly bits. We don?t really know the origins of that. One story says a competitor in a running race tripped over something he was wearing, and after that they decided everyone should go naked,? D?Angour said.

?I think it was to do with a celebration of the body beautiful. They were keen on the beauty of the bodies, shining, oiled bodies with fantastic musculature and beautiful balance,? he said.

Get the latest results from NBCOlympics.com

But overall D?Angour said he thought that any Ancient Greeks transported to London 2012 be pleasantly surprised.

?The ambition to do well, the striving to achieve excellence in a sport ? Let?s say they got over the fact they were living in a different century, I think they would find it fairly familiar and would be excited,? he said.

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A diverse community in East London will welcome the world to Britain for the 2012 Olympic Games. Meet residents and hear how they feel about having a huge, world stage in their backyard.

They might be a little bemused by events such as synchronized swimming, he said, but the 100 meters and the world?s fastest man, Usain Bolt, would likely be popular. But even Bolt would be measured against ancient heroes, whose true speed can only be guessed at.

?I think what they would feel is ?this chap [Bolt] is a bloody fast runner? but ? because they didn?t have records --? they would say ?Diagoras,? --? who ran in 426 BC ? ?was pretty good too, I can tell you,?? D?Angour said.

And there might be a few requests for one ancient favorite, chariot racing, to be restored.

?That would be fantastic, wouldn?t it?? D?Angour said. ?Can you imagine? It?d be like Ben Hur all over again.?

More world stories from NBC News:

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Source: http://worldnews.nbcnews.com/_news/2012/07/26/12932649-millionaire-medalists-will-london-2012-remain-true-to-olympic-spirit?lite

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